fopflash plasmids Search Results


m51 super 8× fopflash reporter  (Addgene inc)
94
Addgene inc m51 super 8× fopflash reporter
M51 Super 8× Fopflash Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m51 super 8× fopflash reporter/product/Addgene inc
Average 94 stars, based on 1 article reviews
m51 super 8× fopflash reporter - by Bioz Stars, 2026-05
94/100 stars
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firefly top- or fopflash luciferase plasmids  (Biomol GmbH)
90
Biomol GmbH firefly top- or fopflash luciferase plasmids
Firefly Top Or Fopflash Luciferase Plasmids, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/firefly top- or fopflash luciferase plasmids/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
firefly top- or fopflash luciferase plasmids - by Bioz Stars, 2026-05
90/100 stars
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reporter plasmids 33 mutated tcf-binding site (fopflash)  (Promega)
90
Promega reporter plasmids 33 mutated tcf-binding site (fopflash)
Reporter Plasmids 33 Mutated Tcf Binding Site (Fopflash), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter plasmids 33 mutated tcf-binding site (fopflash)/product/Promega
Average 90 stars, based on 1 article reviews
reporter plasmids 33 mutated tcf-binding site (fopflash) - by Bioz Stars, 2026-05
90/100 stars
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fopflash plasmid  (Merck KGaA)
90
Merck KGaA fopflash plasmid
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Fopflash Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash plasmid/product/Merck KGaA
Average 90 stars, based on 1 article reviews
fopflash plasmid - by Bioz Stars, 2026-05
90/100 stars
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plasmid carrying a minimal c-fos promoter driving luciferase expression (fopflash)  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare plasmid carrying a minimal c-fos promoter driving luciferase expression (fopflash)
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Plasmid Carrying A Minimal C Fos Promoter Driving Luciferase Expression (Fopflash), supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid carrying a minimal c-fos promoter driving luciferase expression (fopflash)/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
plasmid carrying a minimal c-fos promoter driving luciferase expression (fopflash) - by Bioz Stars, 2026-05
90/100 stars
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fopflash reporter plasmids  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare fopflash reporter plasmids
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Fopflash Reporter Plasmids, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash reporter plasmids/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
fopflash reporter plasmids - by Bioz Stars, 2026-05
90/100 stars
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transfection plasmids topflash and fopflash  (Promega)
90
Promega transfection plasmids topflash and fopflash
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Transfection Plasmids Topflash And Fopflash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transfection plasmids topflash and fopflash/product/Promega
Average 90 stars, based on 1 article reviews
transfection plasmids topflash and fopflash - by Bioz Stars, 2026-05
90/100 stars
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pmlz-fopflash reporter plasmids  (Aventis)
90
Aventis pmlz-fopflash reporter plasmids
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Pmlz Fopflash Reporter Plasmids, supplied by Aventis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmlz-fopflash reporter plasmids/product/Aventis
Average 90 stars, based on 1 article reviews
pmlz-fopflash reporter plasmids - by Bioz Stars, 2026-05
90/100 stars
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reporter plasmids fopflash  (Merck KGaA)
90
Merck KGaA reporter plasmids fopflash
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Reporter Plasmids Fopflash, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter plasmids fopflash/product/Merck KGaA
Average 90 stars, based on 1 article reviews
reporter plasmids fopflash - by Bioz Stars, 2026-05
90/100 stars
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topflash/fopflash plasmids  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare topflash/fopflash plasmids
(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by <t>FOPflash-normalized</t> <t>TOPflash</t> luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.
Topflash/Fopflash Plasmids, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash/fopflash plasmids/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
topflash/fopflash plasmids - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


(A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by FOPflash-normalized TOPflash luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.

Journal: Oncotarget

Article Title: Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells

doi: 10.18632/oncotarget.17056

Figure Lengend Snippet: (A) Cell viability of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 24 or 48 h. Data are presented as mean±SD. Experiments were performed in triplicate. (B) Relative TCF/LEF luciferase activity measured by FOPflash-normalized TOPflash luciferase activityin RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. (C) RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 1 h. The mRNA expression of indicated genes was detected by qRT-PCR analysis. (D and E) The expression of TCF4-interacting proteins and phosphorylation of TNIK detected by immunoprecipitation and Western blot of RPMI8226 cells treated with IL-6 (50 ng/mL) and KY-05009 (3 μM) or dovitinib (3 μM) alone or in combination for 9 h. * P < 0.01, * P < 0.001 versus control; # P < 0.01, ## P <0.001 versus cells treated with IL-6 alone.

Article Snippet: RPMI8226 cells were trasfected with TOPflash TCF reporter plasmid (wild-type TCF binding site) (Merck Millipore, Darmstadt, Germany), FOPflash plasmid (mutant TCF binding site) (Merck Millipore, Darmstadt, Germany), and Lipofectamine 2000 (Thermo Fisher Scientific, Boston, MA, USA) in an antibiotic-free medium.

Techniques: Luciferase, Activity Assay, Expressing, Quantitative RT-PCR, Phospho-proteomics, Immunoprecipitation, Western Blot, Control